May 22, 2014

How long will it take?

If you want samples sequenced in a timely manner, we suggest one of these options.

The first thing is to insure that you supply us with high quality DNA/RNA and follow our sample submission instructions.

Then in terms of getting the samples on the sequencer it depends on a number of factors listed below.

The list is roughly in order of time to start a sequence run once samples are delivered.

These rules apply to all types of sequencing runs

  1. MiSeq run (fastest option), PacBio, minION
  2. Full flow cell of HiSeq rapid (2 lanes)
  3. Full flow cell HiSeq High Output 2500 or 4000 (8 lanes)
  4. One lane HiSeq rapid (flexible type (PE or SE and any length)
  5. One lane HiSeq Rapid (specific length and end type) *
  6. More then one lane HiSeq HO (flexible)
  7. More then one lane HiSeq HO (specific length and end-type)
  8. One lane HiSeq HO (specific length and end-type)
  9. Part of a lane (spike-in) (flexible for length and end-type)
    We no longer offer this option because of excessive long term around times
  10. Part of a lane (spike-in) specific length and end-type)
    We no longer offer this option because of excessive long term around times

* Different run types can very considerably in terms of popularity so a run configuration that is less popular will take a long time to fill up.

Most popular Illumina run types

HiSeq 4000 : 150X150

HiSeq 2500v4 : 125X125

Our least popular run types

HiSeq 4000 : 75X75
wait time ∞

HiSeq 2500R: 250X250  This is the only type of run we accept spike ins. For the two HiSeq 2500R lanes we normally combine 10 projects into one flow cell. Each project generates the equivalent of one miSeq run’s worth of data, at a cost 1/2 that of the miSeq.   Wait time: 6 months