November 1, 2016

Sequencing

AgriLIfe Research

Modern DNA Sequencing 

Post-Sanger Sequencing, Next Generation Sequencing (NGS), High Throughput, High Output, 2nd Generation Sequencing, 3rd Generation Sequencing… and the names go on, but the song remains the same. The ability to translate  chemical information embedded in the composition of deoxyribonucleic acid molecules (DNA), faster and cheaper then was thought to be possible only a few short years ago.

Sequencing services we offer (Start a project) (Internal Cost per Lane)

Illumina

Illumina next-generation sequencing (NGS) technology enables rapid, accurate sequencing. It can be used to sequence the DNA from any organism, providing valuable information in response to almost any biological question. It can be applied to small, targeted regions or the entire genome through a variety of methods. When doing a study using the Illumina system we ask that you plan your study such that is uses at least one full lane. Before any sequencing process can start we have to fill the whole flow cell, normally that is done by combining projects (up to 8 projects on a full size flow cell).

NovaSeq

The NovaSeq Series of Sequencing Systems unleashes groundbreaking innovations, including scalable throughput and flexibility for virtually any sequencing method. Currently we are validating and testing, but please contact us if you have a large project that would be able to use a full flow cell (equivalent of 10 HiSeq 4000 lanes).

HiSeq 4000

Offering ultra-high throughput, the HiSeq HiSeq 4000 Systems deliver ~330 million reads/lane. Our most common run type for the 4000 is 150X150 which generates ~94GB/lane. 8 lanes per flow cell.

Available read lengths: 50SE, 75PE, 150SE, and 150PE

HiSeq 2500v4

The HiSeq 2500 System is efficient high-throughput sequencing system that supports a broad range of applications and we have found to be our most robust system. The 2500 generates ~250 million reads per lane in high output mode, and 150M reads per lane in rapid mode. High output mode uses an 8 lane flow cell and rapid mode uses a 2 lane flow cell.

Available read lengths: 50SE, 50PE, 125SE, 125PE, 150PE (rapid mode), 250PE (rapid mode)

MiSeq v3

The MiSeq desktop sequencer is usefull for focused applications such as targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression, amplicon sequencing, reaching up to 15 Gb of output with 25 M sequencing reads and 2×300 bp read lengths.

Available read lengths: 150SE, 300PE

PacBio

Built on Single Molecule, Real-Time (SMRT) technology, the scalable, high-throughput Sequel System delivers unprecedented sequencing results through long reads, uniform coverage, and high consensus accuracy.

Sequel

The Sequel System is based on the Single Molecule, Real-Time (SMRT) technology and delivers about 5X more reads. It provides the following advantages: a) long read lengths, b) high consensus Accuracy, c) and uniform coverage. Applications; Whole Genome Sequencing, Targeted Sequencing, Whole Transcript, Metagenomics, Epigenetics.

2016-10-26-13-37-13

 


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