Job ID:
Job date: 2014-12-18
End Date:
Company : Moderna Therapeutics Inc. Country : Role : Research Scientist
Job date: 2014-12-18
End Date:
Company : Moderna Therapeutics Inc. Country : Role : Research Scientist
Job Description:
We are seeking an innovative and motivated scientist, with hands-on experience in high throughput plasmid cloning, sequencing and sequence analysis, to join the newly formed nucleic acid synthesis team. Able to function in a highly collaborative and fast-paced environment, the successful applicant will support gene synthesis efforts to advance Moderna's mRNA technology platform.
Responsibilities
•Independently perform high throughput cloning, which include restriction-mediated cloning, Gibson cloning, infusion cloning and other next generation cloning methods to clone chemically synthesized genes.
•Pick and grow cloned bacterial colonies for overnight culture and perform high throughput plasmid extraction using state-of-the-art robotics.
•Prepare bacterial glycerol stocks for each construct for long-term storage.
•Using high throughput format, perform plasmid purification for Sanger sequencing.
•Perform sequence analysis using sequence analysis software such as Sequencher and other commercially available sequence analysis platform.
•Construct plasmid maps using Vector NTI software.
•Design sequencing primers for Sanger Sequencing.
•Perform high throughput biochemistry using end-to-end automation involving Robots such as Hamilton, Qpix, LabchipGx and other custom made Robots for PCR assembly and plasmid prep.
•Collaborate with other members of the synthesis team and broadly with other relevant departments within the company to deliver constructs within the specified time-frame.
•Retrieve sequence files from third party vendor and archive Sanger sequencing trace files for each project. Analyze sequence data and provide project updates at regular intervals.
Minimum Qualifications
•BS with at least 5-7 years of industry experience or MS with at least 3 years of industry experience performing high-throughput cloning and sequence analysis.
•Extensive experience in sequence analysis, construction of vector map and primer design is central to this position.
•Hands on experience using robotics such as Hamilton, Qpix, LabchipGX, BioXP3200 for PCR assembly, cloning, colony picking, plasmid extraction and plasmid purification.
•Experience with a variety of standard molecular biology techniques (e.g. PCR, gel electrophoresis, restriction digestion, cloning, vector preparation, bacterial transformation and nucleic acid purification).
•Knowledge and hand-on experience in bioinformatics is highly desirable.
•Excellent interpersonal and collaborative skills, and the ability to work independently and effectively in a highly dynamic environment.
Preferred Qualifications
•Experience with high throughput robotic workstations.
•Familiarity with nucleic acid therapeutics.
•Ability to multi-task to meet technology development and research goals.
Competencies
•Effectively prioritize work
•Ability to execute in a fast pace environment
•Teamwork
•Learning Agility
•Effective Communication (clear verbal and written communication)
Why Join Moderna
Moderna is pioneering messenger RNA Therapeutics™, an entirely new in vivo drug modality that produces human proteins or antibodies inside patient cells, which are in turn secreted or active intracellularly. This breakthrough platform addresses currently undruggable targets and offers a superior alternative to existing drug modalities for a wide range of disease conditions. Moderna has developed a broad intellectual property estate, including more than 250 patent applications covering novel nucleotide chemistries and drug compositions. The company plans to develop and commercialize its innovative mRNA drugs through a combination of strategic relationships as well as newly formed ventures.
Founded in late 2010 by Flagship VentureLabs, Cambridge-based Moderna is privately held and currently has strategic agreements with AstraZeneca and Alexion Pharmaceuticals.
Moderna recently signed a collaboration agreement with Astra Zeneca that provided a $240MM upfront payment (plus milestone payments).
Moderna is committed to equal employment opportunity and non-discrimination for all employees and qualified applicants without regard to a person’s race, color, gender, age, religion, national origin, ancestry, disability, veteran status, genetic information, sexual orientation or any characteristic protected under applicable law. Moderna will make reasonable accommodations for qualified individuals with known disabilities, in accordance with applicable law.
Requirements
Please see Job Description
Additional Info:
[Click Here to Access the Original Job Post]
We are seeking an innovative and motivated scientist, with hands-on experience in high throughput plasmid cloning, sequencing and sequence analysis, to join the newly formed nucleic acid synthesis team. Able to function in a highly collaborative and fast-paced environment, the successful applicant will support gene synthesis efforts to advance Moderna's mRNA technology platform.
Responsibilities
•Independently perform high throughput cloning, which include restriction-mediated cloning, Gibson cloning, infusion cloning and other next generation cloning methods to clone chemically synthesized genes.
•Pick and grow cloned bacterial colonies for overnight culture and perform high throughput plasmid extraction using state-of-the-art robotics.
•Prepare bacterial glycerol stocks for each construct for long-term storage.
•Using high throughput format, perform plasmid purification for Sanger sequencing.
•Perform sequence analysis using sequence analysis software such as Sequencher and other commercially available sequence analysis platform.
•Construct plasmid maps using Vector NTI software.
•Design sequencing primers for Sanger Sequencing.
•Perform high throughput biochemistry using end-to-end automation involving Robots such as Hamilton, Qpix, LabchipGx and other custom made Robots for PCR assembly and plasmid prep.
•Collaborate with other members of the synthesis team and broadly with other relevant departments within the company to deliver constructs within the specified time-frame.
•Retrieve sequence files from third party vendor and archive Sanger sequencing trace files for each project. Analyze sequence data and provide project updates at regular intervals.
Minimum Qualifications
•BS with at least 5-7 years of industry experience or MS with at least 3 years of industry experience performing high-throughput cloning and sequence analysis.
•Extensive experience in sequence analysis, construction of vector map and primer design is central to this position.
•Hands on experience using robotics such as Hamilton, Qpix, LabchipGX, BioXP3200 for PCR assembly, cloning, colony picking, plasmid extraction and plasmid purification.
•Experience with a variety of standard molecular biology techniques (e.g. PCR, gel electrophoresis, restriction digestion, cloning, vector preparation, bacterial transformation and nucleic acid purification).
•Knowledge and hand-on experience in bioinformatics is highly desirable.
•Excellent interpersonal and collaborative skills, and the ability to work independently and effectively in a highly dynamic environment.
Preferred Qualifications
•Experience with high throughput robotic workstations.
•Familiarity with nucleic acid therapeutics.
•Ability to multi-task to meet technology development and research goals.
Competencies
•Effectively prioritize work
•Ability to execute in a fast pace environment
•Teamwork
•Learning Agility
•Effective Communication (clear verbal and written communication)
Why Join Moderna
Moderna is pioneering messenger RNA Therapeutics™, an entirely new in vivo drug modality that produces human proteins or antibodies inside patient cells, which are in turn secreted or active intracellularly. This breakthrough platform addresses currently undruggable targets and offers a superior alternative to existing drug modalities for a wide range of disease conditions. Moderna has developed a broad intellectual property estate, including more than 250 patent applications covering novel nucleotide chemistries and drug compositions. The company plans to develop and commercialize its innovative mRNA drugs through a combination of strategic relationships as well as newly formed ventures.
Founded in late 2010 by Flagship VentureLabs, Cambridge-based Moderna is privately held and currently has strategic agreements with AstraZeneca and Alexion Pharmaceuticals.
Moderna recently signed a collaboration agreement with Astra Zeneca that provided a $240MM upfront payment (plus milestone payments).
Moderna is committed to equal employment opportunity and non-discrimination for all employees and qualified applicants without regard to a person’s race, color, gender, age, religion, national origin, ancestry, disability, veteran status, genetic information, sexual orientation or any characteristic protected under applicable law. Moderna will make reasonable accommodations for qualified individuals with known disabilities, in accordance with applicable law.
Requirements
Please see Job Description
Requeriments :
Skills :
Areas :
Additional Info:
[Click Here to Access the Original Job Post]