Job Description:
The mission of the Centre for Plant Biotechnology and Breeding that is involved in research and education in the field of genetics and plant breeding is to gain expertise and knowledge and to develop modern (bio) technologies in the spheres of agriculture and food. The Centre group has developed several methods in the field of plant breeding and introduced a series of molecular marker systems. The group set up a genomic (PCR cyclers, real-time PCR, DNA sequencer, bioinformatics facilities) and proteomic (2-D system, bioinformatics facilities) lab and has experience in maintenance and management of genetic resources (gene banks) in hop, buckwheat, olive and grapevine. The research group also introduced novel methods for plant pathogen diagnostics and has great expertise on analysis of plant-pathogen interactions and molecular variability for distinguishing and identifying varieties (and intra-varietal variability). The most advanced facility needed to carry out the proposed project and that is not available everywhere, includes NGS facility (Ion Proton sequencer) (www.genetika.si).
Within the project proposal, we wish to conduct a plant genotyping study (GBS) using the NGS facilities. The experimental methodology is already involved and analytical tools considered. The researcher would study genetic variation using Ion AmpliSeq™ Targeted Selection Technology, a simple, fast, and affordable solution for sequencing specific plant genes or genomic regions. This technology allows analyzing hundreds of genes simultaneously, such as SNP polymorphisms or regions of interest, as for example those implicated in a particular disease or specific biochemical pathways.
The aim of the proposed research work is the application of GBS technology to support genomics-assisted plant breeding and high-throughput studies of genetic variability. There are two main expected outcomes related to novel applications of GBS technology; 1) identification of sequence polymorphisms of hops (Humulus lupulus L.) and covering a much higher proportion of the genome than previous marker technologies and revealing patterns of genomic polymorphism linked to traits of agronomic importance; screening thousands of polymorphisms can thus identify a full range of evolutionary events, such as drift, selection and recombination. 2) saturation of current genetic map of hops using F1 mapping population segregating for verticilium wilt tolerance, chemical composition and yield components, 3) the development of improved and efficient methods for detecting, identifying and robustly genotyping sequence polymorphisms with easy transformation to other species.
Sub Research Field: Biotechnology and genetics (NGS Molecular Marker Technologies)
Supervisor: Nataša Stajner, Asoc.prof.
Additional Info:
[Click Here to Access the Original Job Post]
The mission of the Centre for Plant Biotechnology and Breeding that is involved in research and education in the field of genetics and plant breeding is to gain expertise and knowledge and to develop modern (bio) technologies in the spheres of agriculture and food. The Centre group has developed several methods in the field of plant breeding and introduced a series of molecular marker systems. The group set up a genomic (PCR cyclers, real-time PCR, DNA sequencer, bioinformatics facilities) and proteomic (2-D system, bioinformatics facilities) lab and has experience in maintenance and management of genetic resources (gene banks) in hop, buckwheat, olive and grapevine. The research group also introduced novel methods for plant pathogen diagnostics and has great expertise on analysis of plant-pathogen interactions and molecular variability for distinguishing and identifying varieties (and intra-varietal variability). The most advanced facility needed to carry out the proposed project and that is not available everywhere, includes NGS facility (Ion Proton sequencer) (www.genetika.si).
Within the project proposal, we wish to conduct a plant genotyping study (GBS) using the NGS facilities. The experimental methodology is already involved and analytical tools considered. The researcher would study genetic variation using Ion AmpliSeq™ Targeted Selection Technology, a simple, fast, and affordable solution for sequencing specific plant genes or genomic regions. This technology allows analyzing hundreds of genes simultaneously, such as SNP polymorphisms or regions of interest, as for example those implicated in a particular disease or specific biochemical pathways.
The aim of the proposed research work is the application of GBS technology to support genomics-assisted plant breeding and high-throughput studies of genetic variability. There are two main expected outcomes related to novel applications of GBS technology; 1) identification of sequence polymorphisms of hops (Humulus lupulus L.) and covering a much higher proportion of the genome than previous marker technologies and revealing patterns of genomic polymorphism linked to traits of agronomic importance; screening thousands of polymorphisms can thus identify a full range of evolutionary events, such as drift, selection and recombination. 2) saturation of current genetic map of hops using F1 mapping population segregating for verticilium wilt tolerance, chemical composition and yield components, 3) the development of improved and efficient methods for detecting, identifying and robustly genotyping sequence polymorphisms with easy transformation to other species.
Sub Research Field: Biotechnology and genetics (NGS Molecular Marker Technologies)
Supervisor: Nataša Stajner, Asoc.prof.
Skills :
Areas :
Additional Info:
[Click Here to Access the Original Job Post]